Hansenula capsulata phosphonomannan

In addition to the coupled-signal method just described, phosphorylated carbon signals can be detected by use of praseodymium chloride, which displaces a- and /8-carbon resonances of a,/8-D-mannose 6-phosphate and a-D-mannosyl phosphate downfield, with little effect on other resonances. Europium chloride has analogous properties, except that the displacements are upfield. With certain polysaccharides, such as the O-phosphonomannan of Hansenula capsulata (29), the sig-... 

Mannans of Hansenula capsulata Y-1842 and H. holstii Y-2448 differ structurally from the phosphonomannan components.83 The man-nan of H. capsulata is probably a cell-wall component, and it has the linear, repeating-structure 6. Other Pichia and Hansenula mannans have predominantly linear structures.84 The 13C-n.m.r. spectrum of 6 (see Fig. 2) is distinguishable from that of the branched-chain man-nan85 from Saccharomyces rouxii (repeating unit 7.)... 

To the phosphonomannan of Hansenula capsulata Y-1842 was attributed the principal structure 15, as, on partial hydrolysis, it gave a mannobiose 6-phosphate (16) which provided 1 mol of formalde-... 

O-Phosphonomannan/mannan Hansenula sp. Hansenula capsulata Hansenula holstii Pichia sp. Selenotila peltata... 

The phosphonomannan of Hansenula capsulata has a mannose to phosphate ratio of 2.5 1, which is half that of the H. holstii polymer. It consumed 1.03 moles of sodium periodate with formation of 0.31 mole of formic acid per mole of mannose residue. Autohydrolysis of the phosphonomannan gave 2-0-(6-0-phosphono-a-D-mannopyrano-syl)-D-mannose and a trisaccharide which probably has the structure depicted in (12). The cross reaction of antityphoid horse serum with the phosphonomannan of H. holstii also provides evidence for the presence of (1— 2)- or (1— 3)-linked D-mannopyranose residues, or both. ... 

Figure 3. Repeat units of poly (phosphoric diester) O-phosphonomannans the unit depicted for Hansenula capsulata is a trimer...

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