Hansenula capsulata

In addition to the coupled-signal method just described, phosphorylated carbon signals can be detected by use of praseodymium chloride, which displaces a- and /8-carbon resonances of a,/8-D-mannose 6-phosphate and a-D-mannosyl phosphate downfield, with little effect on other resonances. Europium chloride has analogous properties, except that the displacements are upfield. With certain polysaccharides, such as the O-phosphonomannan of Hansenula capsulata (29), the sig-... 

Mannans of Hansenula capsulata Y-1842 and H. holstii Y-2448 differ structurally from the phosphonomannan components.83 The man-nan of H. capsulata is probably a cell-wall component, and it has the linear, repeating-structure 6. Other Pichia and Hansenula mannans have predominantly linear structures.84 The 13C-n.m.r. spectrum of 6 (see Fig. 2) is distinguishable from that of the branched-chain man-nan85 from Saccharomyces rouxii (repeating unit 7.)... 

Fig. 2,— 13C-N.m.r. Spectrum of Mannan from Hansenula capsulata in DaO. (Numerical values are chemical shifts, 8C.)...

To the phosphonomannan of Hansenula capsulata Y-1842 was attributed the principal structure 15, as, on partial hydrolysis, it gave a mannobiose 6-phosphate (16) which provided 1 mol of formalde-... 

Phosphomannane Hansenula capsulata Hansenula holstii Thixotrophic at high contration, gels with borax... 

O-Phosphonomannan/mannan Hansenula sp. Hansenula capsulata Hansenula holstii Pichia sp. Selenotila peltata... 

Hansenula capsulata, Hansenula minuta, Pachysolen tannophilus, Torulopsis pinus, and Saccharomyces pirn have been patented. 

The phosphonomannan of Hansenula capsulata has a mannose to phosphate ratio of 2.5 1, which is half that of the H. holstii polymer. It consumed 1.03 moles of sodium periodate with formation of 0.31 mole of formic acid per mole of mannose residue. Autohydrolysis of the phosphonomannan gave 2-0-(6-0-phosphono-a-D-mannopyrano-syl)-D-mannose and a trisaccharide which probably has the structure depicted in (12). The cross reaction of antityphoid horse serum with the phosphonomannan of H. holstii also provides evidence for the presence of (1— 2)- or (1— 3)-linked D-mannopyranose residues, or both. ... 

Spectroscopic Properties.— The phosphomannan of the yeast Hansenula capsulata is too viscous for spectroscopic studies. Partial hydrolysis, however, affords a phosphate of 2-0-j3-D-mannopyranosyl-a,/5-D-mannose which has been studied. From C-0- P couplings in the C n.m.r. spectrum of this phosphate and a comparison with the spectrum of the dephosphorylated mannobiose, the structure of the phosphate was deduced to be (10). This has... 

Racemic estradienes, e.g., 2. are selectively reduced by microorganisms302. In reductions carried out by Hansenula capsulata and Saccharomyces chevalieri. only the naturally configurated enantiomer is reduced, whereas the other enantiomer remains unchanged. 

Figure 3. Repeat units of poly (phosphoric diester) O-phosphonomannans the unit depicted for Hansenula capsulata is a trimer...

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