Hamster antibody production test

Hamster antibody production test (HAP) a broad-screen test for known viruses potentially present in test article (for rAHF-PFM cell line only). 

The absence of detectable response to MBS A in the mouse is not surprising, since Plescia and Braun (1967) have reported that this protein is not antigenic in this rodent. Use of the technique of Farr (1958) allowed a comparison of the production of antibodies against poly I poly C in mice and rabbits immunized with the same preparation of poly I poly C — MBS A (Fourcade et al., manuscript in preparation). The average percentage of poly I poly C bound by the immune sera was 14 (xg/ml antiserum in mice and 21 fig/ml antiserum in rabbits. The hamster displays the same responses to carrier protein as the mouse, and the antibodies induced react with poly I poly C but not with MBSA, when tested by immunodiffusion. 

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