Halobacterium salinarum strain

For photo taxis assays, the cells are grown to the late exponential phase, diluted 100-fold in fresh medium, and grown for 2 h for use in motility measurements. The phototaxis response that will be observed depends critically on the strain of Halobacterium salinarum that is used see Note 1). 

It is essential to utilize C- or N-labeled proteins or peptides, instead of natural abundance, as samples to be used for solid-state NMR, in order to improve the sensitivity and/or selectivity of the amino acid residues under consideration. Such isotopically labeled bR samples needed for NMR studies are readily available from the large-scale culture of Halobacterium salinarum S-9 strain using synthetic media in which certain unlabeled amino acids are replaced with Relabeled amino acid species such as [3- RC]Ala or [l- RC]Val. This is shown in the circled or boxed residues, respectively, in the schematic representation of the primary sequence of bR, taking into account the secondary folding on the basis of X-ray diffraction, in Fig. 4. Undoubtedly, selective isotope labeling in this way, although not site-directed isotope labeling, could be most favorable in... 

Gruber, C., Legat, A., Pfalfenhuemer, M., Radax, C., Weidler, G., Busse, H J., Slan-Lotter, H. Halcbacterium noricense sp. nov., an archaeal isolate from a bore core of an alpine Permian salt deposit, classification of Halobacterium sp. NRC-1 as a strain of H. salinarum and emended description ofH. salinarum. Extremophiles 2004,8(6), 431 39. 

---