Guanosine selective oxidation

Joffe A, Geacintov NE, Shafirovich V (2003) DNA lesions derived from the site selective oxidation of guanine by carbonate radical anions. Chem Res Toxicol 16 1528 1538 Masuda M, Suzuki T, Friensen MD, Ravanat JL, Cadet J, Pignattelli B, Nishino H, Ohshi-ma H (2001) Chlorination of guanosine and other nucleosides by hypochlorous acid and myeloperoxidase of activated human neutrophils catalysis by nicotine and trimethylamine. J Biol Chem 276 40486 10496... 

De Oliveira, J. E, Teixeira, C. E., Arantes, E. C. et al.. Relaxation of rabbit corpus cavemosum by selective activators of voltage-gated sodium channels role of nitric oxide-cyclic guanosine monophosphate pathway. Urology 62, 581, 2003. 

Electrocatalytic oxidation of guanosine residues by electrogenerated tris(2,2 -bipyridyl)ruthenium(III) has recently been shown to provide a useful transduction mechanism in DNA biosensors [212). Probe strands in which inosine was substituted for guanosine were immobilized on an electrode surface, and these hybridized selectively with targets that contained oxidizable guanosine residues PCR-amplified genomic DNA from herpes simplex virus type II, Clostridium perfrin-gens, and human immunodeficiency virus were detected by this method. 

CILE over CPE with IL as the modifier. The presence of IL showed the adsorptive ability and had good promotion to the oxidation of guanosine. Under the selected conditions a new electrochemical method for guanosine detection was further established with the detection limit of 2.61 X 10 mol L" (3 o) by cyclic voltammetry and successfully applied to the human urine sample determination. 

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