Growth factors expressed mesenchymal stem cells

The rate of mesenchymal stem cell (MSC) proliferation in monolayer environment is slow, a problem which needs to be addressed in view of the need to obtain sufficient number of cells within a reasonable time frame./n vitro growth and activities of MSCs are supported and enhanced by biochemical such as cell culture medium. These include-growth factors, cytokines, and hormones.However,it is recently reported that mechanical forces also play acentral role in the physiological process involving a wide variety of tissues [6-9]. Cyclic uniaxial strain applied on elastic substrates causes changes in cell responses, orientation and alignment.The application of mechanical force also influences cell proliferation, differentiation, and gene expression in a wide variety of cell types [10-12]. 

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