Granulosa cell aromatase bioassay for FSH

The study of potential paracrine modulators (estrogens, androgens and growth factors) that could enhance FSH action led to the development of an in vitro granulosa cell aromatase bioassay (GAB). The combined action of enhancing hormones and factors resulted in an assay highly sensitive to the aromatase-inducing function of FSH [69], This assay is hormone-specific and sensitive, and has been applied to the measurement of FSH bioactivity in serum [70] and urine samples [71] from humans and diverse animal species. [Pg.188]

Induction of cholesterol side-chain cleavage enzymes [Pg.188]

The rate-limiting step in progestin biosynthesis is the side-chain cleavage of cholesterol to pregnenolone taking place in the mitochondria. At least three steps in the cholesterol side-chain cleavage (SSC) reaction may be under hormonal regulation [72,73]. FSH stimulates the synthesis of the SSC cytochrome P-450 which results in the stimulation of SSC activity [74]. [Pg.188]

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