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Gradient elution chromatography VERSE model

Extension of the VERSE Model to Nonlinear Gradient Elution Chromatography 724... [Pg.699]

The VERSE method was extended to describe the consequences of protein denat-uration on breakthrough curves in frontal analysis and on elution band profiles in nonlinear isocratic and gradient elution chromatography [69]. These authors assumed that a unimolecular and irreversible reaction taking place in the adsorbed phase accoimts properly for the denaturation. The choice of this reaction was based on numerous literature observations of the irreversible adsorption of proteins on hydrophobic surfaces [70]. This model (VERSE-LC) was verified using... [Pg.774]

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