Good’s buffers

Differences in electrophysiological and contractile properties of mammalian cardiac tissues bathed in bicarbonate- and HEPES-buffered solutions, Acto Physiol. Scand.YT, 11-18, 2003 Mash, H.E., Chin, Y.P., Sigg, L. et al., Complexation of copper by zwitterionic aminosulfonic (good) buffers. Anal. Chem. 75, 671-677, 2003 Sokolowska, M. and Bal, W., Cu(ll) complexation by non-coordinating M2-hydroxyethylpiperazine-iV -ethanesulfonic acid (HEPES buffer), J. Inorg. Biochem. 99, 1653-1660, 2005 Zhao, G. and Chasteen, N.D., Oxidation of Good s buffers by hydrogen peroxide, Anal. Biochem. 349, 262-261, 2006 Hartman, R.F. and Rose, S.D., Kinetics and mechanism of the addition of nucleophiles to alpha,beta-unsaturated thiol esters, J. Org. Chem. 71, 6342-6350, 2006. 

The choice of buffer depends above all on the required pH value. Then, consideration needs to be given to whether the buffering substance will interfere with the experiment in any way. Cost factors also play a role acetate, phosphate and Tris buffers are cheap, whereas Good s buffers (Table 2-2) are relatively expensive. All buf-... 

Biuret Cu2+ reaction with peptide bonds 1-20 mg NH4+, Tris, Good s buffers Rapid insensitive... 

Substances interfering with this assay include buffers which are amino acid or peptide in nature, such as Tris and Good s buffers, because they give positive color formation. The Cu ions are also susceptible to reduction. This can be detected in practice by appearance of a red precipitate in the reaction mixture. 

Taurine is an essential dietary nutrient in felines and is routinely added to packaged food for domestic cats. Other aminoalkanesulfonic acids, e.g., A-2-hydroxyethyl-piperazine-A -2-ethanesulfonic acid (HEPES), 2-[N-morpholino]ethanesulfonic acid (MES), or 3-(n-mor-pholino)propanesulfonic acid (MOPS), are commonly referred to as biological buffers or as Good s buffers. 

Good s Buffers were selected by Dr. Norman Good because they display characteristics making them integral to research in biology and biochemistry (e.g., pK... 

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