Gonadotropins purification

Concanavalin A-agarose has been used in the purification of the glycoprotein hormones, chorionic gonadotropin, and follicle stimulating and leuteinizing hormones [129]. Delipidated rat brain glycoproteins in 1% sodium deoxycholate can be purified on Con A-agarose [130]. 

Extraction and Purification of Pituitary Gonadotropins from Urine and Plasma 13... 

Problems in relation to the purification of the gonadotropins depend essentially on the properties of the contaminants, and, accordingly, the... 

Zinc Precipitation. The initial stage in Courrier s purification of LH and FSH from the urine of postmenopausal women is the precipitation of all the gonadotropins from urine with 0.02 Af zinc acetate... 

Much of the work on urinary gonadotropins has been concerned with the separation of LH from FSH rather than with purification of either of the hormones. Several systematic studies have been published describing the use of various types of chromatography and electrophoresis in the separation of the urinary gonadotropins, and there is now general agreement that it is somewhat easier to prepare LH relatively free from FSH than vice versa. Nevertheless, urinary FSH has now been obtained in a more highly purified form than has urinary LH. 

Courrier, R., Sur la purification de I hormone folliculo-stimulante h3rpophysaire (F.S.H.) de mouton et de la gonadotropine urinaire humaine de menopause (H.M.G.). Acta Endocrinol. (Copenhagen), Suppl. 90, 29-33 (1964). 

D2. Donini, P., Puzzuoli, D., D Alessio, I., Bergesi, G., and Donini, S., Purification and properties of urinary foUicle-stimulating and luteinizing hormones. In Gonadotropins, Proc. Workshop Conf. (E. Rosemberg, ed.), pp. 37-41. Geron-X, Los Altos, California, 1968. 

Ota, M., Dronkert, A., and Obara, K., Further purification and characterization of the gonadotropin-inhibiting substance in human urine. Proc. 7th Int. Congr. Biochem., Tokyo Abstr. V, G32, p. 844 (1967). 

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