Golgi mannosidase

Herscovics, A.,Schneikert, J., Athanassiadis, A., and Moremen, K. W. (1994). Isolation of a mouse golgi mannosidase cDNA, a member of a gene fairy conserved from yeast to mammals. J. Biol. Chem., 269, 9864-9871. 

Tulsiani DRP, Harris TM, Touster O. Swainsonine inhibits the biosynthesis of complex glycoproteins by inhibition of Golgi mannosidase-11. J. Biol. Chem. 1982 257(14) 7936-7939. 

Tulsiani, D.R.P., Broquist,H.P., and Touster, 0. (1985). Marked differences in the swainsonine inhibition of rat liver lysosomal Ot-D-mannosidase, rat liver Golgi mannosidase II, and jack bean Oi-D-mannosidase. Arch. Biochem. Biophys., 236, 427-434. 

The transcripts encoding both Golgi mannosidase lA and IB were detected in Northern blots from most mouse [118], rat [110], and human [119] tissues, but there were significant differences in the ratios of the two transcripts. Differences in expression at the cellular level were also detected by immunofluorescence in the rat reproductive tract [120]. These data indicate that the similar Golgi mannosidase 1-like forms are most likely not redundant enzymes but that ancestral gene duplication and divergence has resulted in tissue-specific expression patterns. 

A mouse model for HEMPAS disease was created by the inactivation of the Golgi mannosidase II gene and many of the characteristics of the human disease were reproduced [70]. The mice show anemia, splenomegaly, and immature erythrocytes or reticulocytes in the peripheral blood. Lectin blots and cell sorting... 

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