Goat counting

Three of the six goats in the high-dose test died before the exposure ended, so useful data were not obtained. All exposures, however, lowered the white-cell count and the hematocrit. No change in blood-gas contents was seen. No significant changes in biochemical or pathologic factors were found.1 ... 

Figure 2. Inhibition of antibody binding of 3H-lacto-N-difucohexaitol I by oligosaccharides. Immune goat serum, 10 /d, is mixed with varying amounts of inhibitor in Tris buffer, pH 7.5, containing 0.14 M NaCl, 5 X 10 4 M MgSO(, and 1.5 X 10 4 CaClt in a final volume of 300 id. After incubation for 20 min at 37°, 6.7 pmoles of 3H-lacto-N-difucohexaitol I (103 cpm) in 100 /d of buffer are added. After a second incubation at 37° for 30 min the mixture is passed through a nitrocellulose filter. The filter is washed with 10 ml of the same buffer and counted in a scintillation counter. In the absence of inhibitor 2200 cpm are bound. When the assay is performed with preimmune serum the filter nonspecifically traps 30 to 40 cpm.

In only a few weeks, that little girl s cheeks were rosy, her energy was back, and she was outside playing with the cats or feeding grass to our goats. Her red blood cell count was back to normal. Soon her parents came and took her back home with them to Canada. 

Routine Blood Tests. Complete blood counts (CBCs) (I), platelet counts (2), plasma hemoglobin (3), and fibrinogen concentration (4) were all done by standard methods modified by us for goat blood. 

Using this technique, approximately 285 xCi of inIn has been injected. The labeling efficiency, defined as the counts per minute (CPM) platelet suspension/total CPM U1ln used, was 48 14% in vivo recoveries (1 h after injection) were 72 15%. Mean platelet survival time in five goats was 118 h (5). 

Figure 3 demonstrates the repeatable as well as nonrepeatable aspects of the model in different goats. In this graph, the ordinate was changed by using the U1ln counts/cm of thrombus recovered from the catheter and the specific radioactivity of the platelets in the blood to convert the count rate to absolute numbers of retained platelets by ... 

Perez-Marin et al. (42) significantly improved these results by using MPLS for regression purposes and SNV and detrending treatments to correct for scatter. Nufiez-Sanchez et al. (36) compared the accuracy of folded transmission in liquid milk and reflectance of dried milk NIR calibration equations to predict quality parameters of ewe s milk. The results obtained for each constituent in both NIR analysis modes showed excellent capacity for quantitative analysis with higher than 0.9. Also, the equation obtained for somatic cell count (SCC) by both methods had adequate accuracy, similar to that obtained for goat s milk by Perez-Marin et al. (42) and much higher than the model reported by Tsenkova et al. (54). 

Bound 80 S and free cytoplasmic polysomes were incubated in an amino-acid incorporating system with [ HJleucine. Labeled, nascent polypeptide chains were released from ribosomes with 0.2% SDS and 50 mM EDTA. After dialysis against 5 mM Tris-Cl (pH 7.5) and 1 mM EDTA, the labeled products were lyophilized and resuspended in 1/5 the in vitro reaction volume. Aliquots were added to a solution containing 0.5% Triton X-100 and 0.5% deoxycholate to which immune or nonimmune rabbit y-globulin was added. After incubation for 2 h at 37°C, goat antiserum to rabbit y-globulin was added, and the reaction was incubated for an additional 1 h at 37°C. After centrifugation, the pellets were washed several times, dissolved in Protosol, and counted. 

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