Glycosaminoglycan-peptide

FIGURE 3.58 Schematic representation of the in situ gelling of poly(ethylene glycol)-peptide and glycosaminoglycan-peptide conjugates by Michael-t5 pe addition. Source Tsurkan et al. [86]. Reproduced with permission from John Wiley Sons. 

VA VanderNoot, RE Hileman, JS Dordick, RJ Linhardt. Affinity capillary electrophoresis employing immobilized glycosaminoglycan to resolve heparin-binding peptides. Electrophoresis 19 437-441, 1998. 

Many compounds such as glycosaminoglycans (GAGs), nucleic acids, acidic peptides, or peptides with acidic PTMs such as phosphorylation or sulfation do not readily form positive ions, especially in mixtures where ion formation is favored by the more basic mixture components. When positive ions can be formed, then the spectra are usually characterized by the abundant loss of the PTM (e.g., sulfate from GAG) or, in the case of oligonucleotides, a proton from the sugar-phosphate backbone. 

Fig. 6.12 Peptide domains and attached glycosaminoglycans in aggrecan and versican. HABR Hyaluronic Acid-Binding Region GAG Glycosaminoglycan-Attachment domain EGF Epidermal Growth Factor-like repeat LEC C-type Lectin-like module C (yellow) Complement regulatory protein-like module (see text) (From GlycoWord website http //www.glycoforum. gr.jp/science/word/proteoglycan/PGA03E.html (contributed by Toshikazu Yada))...

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