Glycoproteins production

Several mucolytics reduce the viscosity of mucus by cleaving the disulfide bonds that maintain the gel stmcture. AJ-Acet l-L-cysteine [616-91 -1] (19), introduced in 1963, and mesna [19677-45-5] (20), developed in Europe in the early 1970s (20,21), are effective compounds in this class. Whereas most mucolytics must be adrninistered by aerosol, carbocysteine [638-23-6] (21), which contains a derivatized sulfhydryl group, has shown activity by the oral route (22,23). However, carbocysteine does not reduce mucus viscosity, as does acetylcysteine, but appears to have a direct action on mucus glycoprotein production (24). 

Jarvis, D.L. (2003) Developing baculovirus-insect cell expression systems for humanized recombinant glycoprotein production. Virology, 310 (1), 1-7. 

Fussenegger M, Bailey JE, Hauser H, Mueller PP (1999), Genetic optimization of recombinant glycoprotein production by mammalian cells, Trends Biotechnol. 17 35-42. 

Chico E, Jager V (2000), Perfusion culture of baculovirus-infected BTI-Tn-5Bl-4 insect cells a method to restore cell specific 3-trace glycoprotein productivity at high cell density, Biotechnol. Bioeng. 70 574-586. 

These systems have the shortcoming of yielding glycoproteins in which carbohydrates not seen in humans are partially present. Furthermore, these carbohydrates are not homogeneous. To resolve these problems, attempts have been made to develop new glycoprotein production techniques making use of some other cell expression systems [56]. 

M. Ohta, N. Kawasaki, S. Itoh, T. Hayakawa, Usefulness of glycopeptide mapping by US-MS in comparability assessment of glycoprotein products, Biologicals, 30 (2002) 235. 

CHO Chinese hamster ovary Epithelial Recombinant glycoprotein production... 

The mandate of biotechnology to develop cost effective, robust recombinant glycoprotein production processes with consistent, complete, and physiologically tol-... 

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