Glycoproteins ovalbumin oligosaccharide

The enzymatic synthesis of a hybrid type oligosaccharide on the hen ovalbumin with 13C-enriched galactose allowed the measurement of the 13C-NMR spectra of the whole glycoprotein and additionally, the determination of the correlation times of the protein and the oligosaccharide to 25 ns and 40-80 ns, respectively [168]. This implies that the carbohydrate, at least in its terminal monosaccharide constituents, has a much higher flexibility than the protein, and its flexibility is only little impeded by the attachment to the protein. 

Other soluble glycoproteins such as Unit A glycopeptide of thyroglobulin, Taka-amylase (a microbial o-amylase), and ovalbumin. The structure (23) of the oligosaccharide portion of a low molecular weight lipid-linked intermediate, which is primarily involved in the assembly of a major lipid-linked oligosaccharide of Chinese hamster ovary cells, has been established. ... 

Figure 6.2 MALDI-MS spectra of neutral oligosaccharides obtained by PNGase F digestion from lOOpg of glycoprotein, (a) IgGl (Herceptin), recorded on qQTOF instrument (b) Ovalbumin, recorded on...

Miscellaneous.—An e db-j8-D-acetamidodeoxyglucanase obtained from culture filtrates of Streptomyces griseus rapidly released oligosaccharides from Saccharo-myces cerevisiae )8-fructofuranosidase, bovine pancreatic ribonuclease I and deoxyribonuclease 1, and sulphitolysed ovalbumin these glycoproteins contain a D-mannopyranosyl residue attached to di-iV-acetylchitobiose, which, in turn, is linked to an L-asparagyl residue. Analysis of the hydrolysis products showed that the enzyme cleaved between the 2-acetamido-2-deoxy-D-glucopyranosyl residues. 

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