Oligosaccharides hybrid type

The enzymatic synthesis of a hybrid type oligosaccharide on the hen ovalbumin with 13C-enriched galactose allowed the measurement of the 13C-NMR spectra of the whole glycoprotein and additionally, the determination of the correlation times of the protein and the oligosaccharide to 25 ns and 40-80 ns, respectively [168]. This implies that the carbohydrate, at least in its terminal monosaccharide constituents, has a much higher flexibility than the protein, and its flexibility is only little impeded by the attachment to the protein. 

L. M. Likhosherstov, V. E. Piskarev, N. F. Sepetov, V. A. Derevitskaya, and N. K. Kochetkov, Structure of the oligosaccharide chains of riboflavin-binding glycoprotein from hen egg white. 4. Neutral oligosaccharides of a hybrid type, Bioorg. Khim., 17 (1991) 246-251. 

Hence, this antibody did not contain high mannose or hybrid type oligosaccharides. 

Figure 6. High-mannose and hybrid-type N-linked oligosaccharide chains.

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