Glycoproteins cross-linkers

On the other end of the cross-linker, the hydrazide functional group can react with periodate-oxidized carbohydrate molecules to form hydrazone linkages (Chapter 1, Sections 2 and 4.5). Thus, glycoproteins can be targeted specifically at their polysaccharide chains, avoiding cross-linking at active sites which can lead to activity losses (Fig. 167). 

The ethylene diamine—dextran derivative may be used to couple with carboxylate-containing molecules by the carbodiimide reaction, to couple amine-reactive probes, or to further modify using heterobifunctional cross-linkers. The hydrazide—dextran derivative may be used to cross-link aldehyde-containing molecules, such as oxidized carbohydrates or glycoproteins. 

Yuan W, Bazick J, Sodroski J (2006) Characterization of the multiple conformational states of free monomeric and trimeric human immunodeficiency virus envelope glycoproteins after fixation by cross-linker. J Virol 80 6725-6737... 

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