Hydroxyproline-rich glycoprotein extraction

Several of the hydroxyproline-rich glycoproteins extracted from plant tissues have carbohydrate-binding activity these glycoproteins have the characteristics of lectins. These lectins or lectin-like glycoproteins have compositions which are similar to that of the cell wall hydroxyproline-rich glycoprotein. 

The basic extensins. These are hydroxyproline-, lysine- and tyrosine-rich glycoproteins consisting of rigid molecular rods about 80 nm long (14,15), bearing short mono- to tetrasaccharide side-chains (2,14). When newly secreted they bind ionically to the acidic polysaccharides of the cell wall and can be extracted with cold salt solutions later they become much more resistant to salt-extraction and are said to be covalently bound, probably via dimerization of their tyrosine residues to form isodityrosine (15). 

E. Extraction and Analysis of Hydroxyproline (Hyp)-Rich Glycoprotein(s) (Extensin)... 

E. EXTRACTION AND ANALYSIS OF HYDROXYPROLINE (HYP)-RICH GLYCOPROTEIN(S) (EXTENSIN)... 

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