Glycopeptides carbohydrate identification

Synthesis strategies have also been developed to obtain glycopeptide libraries in order to facilitate the investigation of glycoprotein-mediated cell-cell interaction in biological recognition events or the identification and analysis of the interaction between carbohydrates and their receptors. 

FIGURE 103 Identification of a glycopeptide produced by peptic digestion of a monoclonal antibody using accurate mass measurements (the peptide mass matches two possible iso-baric fragments from the protein sequence, and application of CAD fails to provide useful sequence information due to the presence of a labile carbohydrate chain). The identity of this peptide is unequivocally established using accurate mass measurements with Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS).TOFMS,time-of-flight mass spectrometry. 

As a result of the identification of some amino-acid sequences of the two major component polypeptides of the hepatitis B surface antigen, it has been proposed that the antigen contains a single major immunogenic peptide component (mol. wt. 2.2 x 10 ), part of which is modified by the addition of carbohydrate to give rise to a glycopeptide (apparent mol. wt. 2.8 x 10 ). ... 

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