Glycopeptide arrays

Here, we present an overview of the aspects of the design, synthesis, analysis, and screening of combinatorial solid-phase glycopeptide libraries as compared to parallel glycopeptide arrays. 

PARALLEL AND LIBRARY SYNTHESIS OF GLYCOPEPTIDES 14.7.1 Parallel Synthesis of Glycopeptide Arrays... 

While parallel synthesis of arrays of glycopeptides is readily achieved by implementation of the building-block approach (Scheme 14.1, Strategy 2),101 glycopeptide library synthesis in a combinatorial manner via the split-mix method has yet to prove routine. The difficulty lies in the structural analysis of the vast number of compounds generated in picomolar quantities on a single bead. Whereas peptides on... 

Fig. 3.3. Schematic illustration of the proposed architecture of mucus glycoproteins, (a) Subunits constitute a linear array of oligosaccharide clusters interspersed with naked stretches of protein. (b) Trypsin digestion affords glycopeptides corresponding to the oligosaccharide clusters. (c) The whole mucins are formed by an end-to-end association of subunits via disulphide bonds, (d) The hydrodynamic model of mucus glycoproteins conforms to a random coil within a spheroidal solvent domain. (After Allen et al., 1984.)...

In the fabrication of bead-based glycan arrays, glycopeptides and bovine serum albumin neoglycoproteins were conjugated to the carboxyl-presenting beads using water-soluble carbodi-imides. The beads were spatially arrayed into microwells for... 

In an elegant and different approach, an array of C-linked, glycopeptide-like mimics of SLex were synthesized in parallel by an Ugi four-component reaction. Reaction of different anomeric two- or three-carbon extended C-glycosyl aldehydes or acids with resin-bound amine, isonitriles and with other acids or aldehydes, respectively, yielded an array of C-linked analogues. The method is easy to perform in good yield on solid phase. How-... 

---