Glycolipid lymphoma cell

Radiolabeling and the Analysis of Thy-1 Active Glycolipids. The amount of Thy-1 active glycolipids in brain, thymocytes or lymphoma cells was found to be extremely small and could only be detected by immunological methods and not by chemical means. 

This problem was approached by incorporating radioactive percur-sors into the glycolipids of both brain and lymphoma cells of the Thy-1.2 and 1.1 types. We have used C-palmitate as a per-cursor of ceramide, and - C-N-acetylmannosamine as a percursor of sialic acid (7). Glycolipids were isolated and the radioactive gangliosides were resolved by two-dimensional thin layer chromatography in two different solvent systems followed by autoradiography. 

BW5147 (Thy-l.1) and S49.1 (Thy-l.2) lymphoma cell lines are of T cell lineage and express Thy-l antigen. These cells were labeled biosynthetically with C-palmitate or - C-ManNAc. Glycolipids from these cells were isolated by extraction, and two-dimensional TI/3 and autoradiography. About 50 spots were observed after palmitate incorporation (Fig. 5). BW5147 glycolipids... 

Lymphoma cells were labeled with [I- CiManNAc in culture. Chromatography was done as in Figure 3 with the extract of 2 X 10 cells. Thy-I glycolipids are indicated (15). 

We have also found that Thy-1.1 migrates slightly faster than Thy-1.2 in solvent system 1. Structural differences between Thy-1.1 and 1.2 presumably result in different mobility in either solvent system. We found lymphoma cell lines to be a good in vitro source of Thy-1 glycolipid after labeling with either [l-- C]palmitate or [1- C] ManNAc. About 30 compounds were labeled with both plamitate and ManNAc in these cell lines. Additional compounds labeled with palmitate were neutral glycolipids. 

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