Glycerides thin-layer chromatography

Gas-Liquid Chromatography. Gas-liquid chromatography (GLC) affords both a qualitative and, if adequate internal standards are used, a quantitative analysis of the products of lipolysis. It is necessary, however, first to isolate the acids by a suitable method and then to inject them as free acids or as esters. The partial glycerides can be isolated by thin-layer chromatography and can also be determined by GLC of suitable derivatives. The acid(s) remaining in the partial glycerides can be identified readily by GLC following transesterification. Jensen and co-workers have utilized these techniques in their studies of lipase specificity (Jensen et al 1964). 

Gunstone ct found that separation of saturated and unsaturated fatty acid glycerides by crystallization at -10° is improved by addition of silver nitrate in methanol to complex with the unsaturated components. Morris reports separation of CIS and trans fatty acids and oxygenated fatty acids by thin-layer chromatography on silica gel impregnated with silver nitrate. With untreated silica there was no separation. 

Barrett, C. B., M. S. J. Dallas, and F. B. Padley The separation of glycerides by thin-layer chromatography on silica impregnated with silver nitrate. Chem. Ind. 1962, 1050. 

Analytical Procedures. Glyceride fractions from purification of reaction mixtures were analyzed by thin layer silica gel chromatography (TLC) and HPLC. In addition, several preparative TLC s were performed on the isolated triglycerides. The purified triglycerides were esterified directly to methyl esters by a modification of the method of Morrison and Smith (19), and the fatty acid profile determined by gas chromatography using conventional techniques described elsewhere (10). 

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