Glycerides quantitation

Lipoproteins. The lipid moiety of lipoproteins is quite variable both qualitatively and quantitatively. The a-lipoprotein of serum contains glyceride, phosphatide and cholesterol to about 30 -40% of the total complex. The -lipoprotein of serum contains some glyceride but the phosphatide and cholesterol account for nearly 75% of the total. 

Gas-Liquid Chromatography. Gas-liquid chromatography (GLC) affords both a qualitative and, if adequate internal standards are used, a quantitative analysis of the products of lipolysis. It is necessary, however, first to isolate the acids by a suitable method and then to inject them as free acids or as esters. The partial glycerides can be isolated by thin-layer chromatography and can also be determined by GLC of suitable derivatives. The acid(s) remaining in the partial glycerides can be identified readily by GLC following transesterification. Jensen and co-workers have utilized these techniques in their studies of lipase specificity (Jensen et al 1964). 

In quantitative terms, the total fatty acid content of 10 marine fungi tested ranged from around 2 to 10% of tissue dry weight [58]. The glyceride fraction reached 5.1% for Corollospora maritima Werdermann, as compared to only 0.06% for Zalerion maritimum (Linder) Anastasiou [57]. 

Human Erythrocyte Phosphoglycerides. I. Quantitation of Plasmalogens, Fatty Acids and Fatty Glycerides Biochim. et Biophys. Acta 60 80-89 (1962) Biol. Abstr. 40 21989... 

The complete determination of the structure of a glyceride molecule requires a) a quantitative and structural analysis of the fatty acid mixture, and b) the... 

The case for the participation of intravascular lipolysis by lipoprotein lipase seems to be quite impressive. The quantitative importance of this process for chylomicron removal is, however, less clear. An attempt to arrive at a quantitative estimate has been based on the infusion into a dog, at constant rate over a two hour period, of fatty acid labelled chylomicra. The specific activity of the free fatty acid fraction reached a constant level of around 10% of the infused chylomicron glyceride fatty acids (Fredrickson et al. 1958b). This finding indicates that at most one tenth of the free fatty acids in the plasma are derived from intravascular splitting of chylomicra. If free fatty acids were the sole lipid substance available to the tissues, one would expect that formation from radioactive triglyceri-... 

There are no reliable data available regarding the exact proportion of dietary carbohydrate which is converted to triglycerides in the liver lipogenesis in adipose tissue is probably quantitatively much more important under normal circumstances (Shapiro 1957). As far as is known, no lipoprotein glyceride is liberated from adipose tissue into the plasma. 

The glycerides contained 18 1 at sn which was analogous about 50% of the MGD the acyl-labeled DAG quantitatively conve it was subsequently desaturated iii vitro chloroplast membrane... 

Complete resolution of individual phosphatides of animal origin may require conversion to other molecular species. For example, the characterization of phosphatidylcholine, after preliminary separation from other phosphatides, is carried out by enzymatic hydrolysis to form the diacylglycerol, which is derivatized to form the benzoate ester. Re-versed-phase HPLC is then adequate to completely separate the various diacylbenzoyl-glycerides. Internal standards are recommended, since neither the hydrolysis nor the derivatization is necessarily quantitative (209). 

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