Glycans representation

Fig. 2.4 Biosynthesis of gel-forming mucins. Schematic representation of the major steps occurring during the biosynthesis of mucins. Mucin biosynthesis is a sequential process that starts in the endoplasmic reticulum and ends in the trans-compartments of the Golgi complex. Formation of disulfide-linked oligomers/multimers involves two steps dimerization in the endoplasmic reticulum and interdimeric disulfide bonding in the Golgi complex. O-Glycosy-lation of dimeric precursors results in more extended but still flexible chains. See text for further details. Rectangles indicate the NH-terminal D-domains while circles and the bars represent the COOH-terminal CK-domains and the O-glycan chains, respectively...

Figure 9.4 Schematic representation of the macromolecular architecture of a large gel-forming respiratory mucin. Two mucin subunits, each about 500 nm in length, are joined end to end via disulfide bonds (S-S) and consist of oligosaccharide-rich regions (represented by the thickened line) and folded domains stabilized by disulfide bonds (represented by the knots). An expanded portion of one of the oligosaccharide-rich regions (not drawn to scale) shows the variety and density of the attached O-linked glycans...

Fig. 3. An example of a schematic representation of the GMF procedure for determining a glycan sequence, based on the theoretical fragments by GlycosidlQ platform for the heptasaccharide. This figure is adapted from ref. 27.

Fig. 18. The first reverse glycoblotting protocol reported in ref. 97. (A) Schematic representation, showing the procedure for glycoproteomics, profiling both the glycan heterogeneity and sites of glycosyla-tion. (B) General conditions for selective oxidation and reverse glycoblotting of the terminal sialic acids. This figure is adapted from ref. 97. (Continued)...

Figure 2 Immobilization strategies, (a) A schematic representation of noncovalent adsorption. The lipid tail of a neo-glycolipid adheres to a hydrophobic surface via noncovalent interactions, (b) Strategies used for covalent immobilization of glycans.

Sahoo SS, Thomas C, Sheth A, Henson C, York WS. GLYDE-an expressive XML standard for the representation of glycan structure. Carbohydr. Res. 2005 340 2802-2807. 

Fig. 2 Three-dimensional structure of FLAP. Left panet. Monomer A is shown in ribbon representabon and in yellow, while monomer B is shown in surface representation in blue. The relative location of the active site, N-terminus, and GPI anchoring site are indicated. Right panel. Modeled structure of the GPI anchor attached to the 3D structure of PLAP. N-linked complex glycan was also modeled at N122 and at N249. This figure was kindly produced and contributed by Dr. Mark R. Wormald, Oxford Glycobiology Institute, University of Oxford, Oxford, UK...

Fig. 5. Nomenclature and symbolic representations of the major subcategories of glycosphingolipids, from the simple (GlcCer and GalCer) to the root structures. All except Mollu and Arthro are present in mammalian cells. The glycan symbols are as in Fig. 8.

Figure 1. Schematic representation of G3-PE013k4accase complex - the ey regions are the glycan residues that serve to anchor the dendritic blocks Protein image obtained from AstexViewer MSD-EBI Database (8)...

Symbol nomenclature. For symbolic representation of glycan linkage for the annotation of mass spectra, the Consortium for Functional Glycomics (http // www.functionalglycomics.org/static/consortium/) agrees that ... 

These forms are commonly used in the representation of glycans, but none of them is particularly suited for computer processing because of the use of special symbols and some remaining ambiguities regarding the ordering of branches. 

The transformation can be slow and complicated with complex antennary glycans. A Web interface at http //www.glycosciences.de/tools/linucs/ is available for the conversion of the extended forms of lUPAC-lUBMB representations into LINUCS. For example, the LINUCS code for the core pentasaccharide is... 

LinearCode (Benin et al 2002) represents a simpler linear representation of glycans that has been recently adapted by The Consortium for Fnnctional Glycomics (http //www.fnnctionalglycomics.org/). For example the core pentasaccharide is represented as... 

Figure 4. Schematic representation of the in trisaccharide outer chain of complex yV-glycans.

Fig. 10 (a) Schematic representation of the core pentasaccharide (in the dashed box) of N-glycans. The green blocks represent the peptide of the glycoprotein and the yellow blocks represent the oligosaccharide assembly that develops from Ssac. (b) The phenyl-tagged core pentasaccharide (Ssac) and the di- and trisaccharide sub-units selected for the gas-phase conformational investigation... 

Figure 1. Schematic representation of HIV-1 depicting oligosaccharides of the envelope glycoprotein gp 120 (A), and binding of high mannose type V-glycans to the macrophage endocytosis receptor (B). (Taken from [1] with permission.)...

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