Glycan fucosylated

Transferrins Carbohydrate Number of Types and number of glycan Fucosylation Bisecting GalNAc Figure... 

Sawa M, Hsu TL, Itoh T et al (2006) Glycoproteomic probes for fluorescent imaging of fucosylated glycans in vivo. Proc Natl Acad Sci USA 103 12371-12376... 

A fourth family of A-glycans are referred to as hybrid glycans. These glycans share structural features of the high mannose and complex-type families. They usually retain two mannoses on the 6-arm of the trimannosyl core whilst complex-type antennae are elaborated on the 3-arm. Hybrid structures are frequentiy bisected and may also be core fucosylated (Fig. 15.1). It should be noted that whilst core fucosylation is common in complex-type, hybrid and truncated glycans, it is rarely found in high mannose glycans. 

Novel Fucosylated A/-glycan Core Structures in Haemonchus contortus... 

Fig. 15.5. Structures of A/-linked glycans from several different species of parasitic nematodes, illustrating both similarities with mammalian glycans (compare with Figs 15.1 and 15.2) and features unique to nematodes (e.g. tyvelose and PC capping and novel core fucosylation). The filarial nematode glycans are believed to be substituted with charged residues, which are not yet characterized.

Ramirez-Soto, D. and Poretz, R.D. (1991) The (l-3)-linked (X-L-fucosyl group of the IV-glycans of the Wistaria floribunda lectins is recognised by a rabbit antiserum. Carbohydate Research 213, 27-36. 

Similar results were recently obtained with a human monoclonal antibody, with KDEL sequences fused to the C-termini of both heavy chains, expressed in tobacco [33]. As observed for the invertase-HDEL fusion, about 90% of the N-linked glycans on this antibody were of the high-mannose type, with 6-9 mannose residues, while a fraction contained the immunogenic P(l,2)-xylose glyco-epitope (Fig. 15.6). However, this antibody was not a(l,3)-fucosylated, a glycan modification occurring in the trans Golgi [34]. 

Seifert, J. Unverzagt, C., Synthesis of a core-fucosylated, biantetmary octasaccharide as a precursor for glycopeptides of complex N-glycans. Tetrahedron Lett. 1996,37, 6527-6530. 

Huang, H.H., Tsai, P.L. and Khoo, K.H. (2001) Selective expression of different fucosylated epitopes on two distinct sets of Schistosoma mansoni cercarial O-glycans identification of a novel core type and Fewis X structure. Glycobiology 11, 395 106. 

Fig. 2. iV-glycans of the hybrid type. A triantennary structure with sialylated Af-acetyllactosamine (LacNAc) building blocks B biantennary structure with sialylated or fucosylated poly-LacNAc building blocks. The N-glycan core structure is written in bold letters... 

Mori, E., Hedrick, J.L., Wardrip, N.J., Mori, T. and Takasaki, S. (1998) Occurrence of reducing terminal N-acetylglucosamine 3-sulfate and fucosylated outer chains in acidic N-glycans of porcine zona pellucida glycoproteins. Glycoconj J, 15,447-456. 

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