Glutenin function

Lindsay, M.P., Skerritt, J.H. 1999. The glutenin macropolymer of wheat flour doughs Structure-function perspectives. Trends Food Sci Technol 10 247-253. 

Figure 7.25. Storage modulus and loss modulus of glutenin at low moisture contents as a function of temperature (Reprinted from Trends Food Sci. Tech., 5, Kokini, Coeero, Madeka, and De Graaf, The development of state diagrams for cereal proteins, pp. 281-288, Copyright...

Sapirstein, H. D., and W. J. Johnson. 1996. Spectrophotometric method for measuring functional glutenin and rapid screening of wheat quality. In Gluten 96. Proceedings of the International Workshop on Gluten Proteins, ed. C. W. Wrigley, 494-497. Melbourne Royal Australian Chemical Institute. 

Let us see how we might apply the approach shown in Figure 12.2 to seek a solution to the problem. In terms of functionality, the greatest effect of the translocation is to eliminate the Glu-B3 locus, coding for LMW-GS, which are more abundant than HMW-GS in a normal wheat (usually by a factor close to three). If we consider that protein from normal wheat has about 40% glutenin, then the loss of the Glu-B3 locus will reduce the glute-nin content to about 30%, assuming that each of the Glu-3 loci contributes equally to the LMW-GS. 

P. Tosi, A. Giovangrossi, R. D Ovidio, F. Bekes, O. Larroque, J. Napier, and P. Shewry, Modification of the low molecular weight (LMW) glutenin composition of transgenic durum wheat effects on glutenin polymer size and gluten functionality. Mol. Breed. 16(2), 113-126 (2005). 

Extraction of Unreduced Glutelins. Protein molecular sizes or size distributions often influence functional properties, necessitating extraction and characterization of native proteins. Various procedures have solubilized and fractionated unreduced wheat glutenin using dilute acetic acid [34], solutions differing in HCl concentration [35-37], or 50% and 70% I-propanol [38]. 

Figure 41. The glass transition temperature of glutenin as a function of moisture content (modified from [258]).

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