Glutamate polyglutamate

HPLC does not give a good response to long-chain derivatives of folate, therefore, conversion of the natural form of folate, polyglutamates, to mono- or di-glutamate is required. The enzyme used is y-glutamylcarboxypeptidase, extracted from chicken pancreas, hog kidney, or to a lesser extent from... 

Additional y-linked glutamates can be added to form tetrahydrofolyl-triglutamic acid (THF-triglutamic -< acid or H4PteGlu3) as shown here or higher polyglutamates... 

Folate polyglutamate synthetase binds ATP, tetrahydrofolate-(oligo)-glutamate, then glutamate sequentially forming an intermediate folate... 

Williams et al. (1955) have shown that an enzyme from B. subtilis can catalyze the transfer of a 7-glutamyl residue from the polyglutamic acid to D-glutamic acid to form 7-glutamylglutamic acid. 

Thus it is now clear that the polyglutamic acids produced by B. subtilis and B. anthracis are entirely poly-7-D-glutamic acid. 

Tho data in Table 9.1 are from an cxporlment involving foly] poly glutamate synthase. The enzyme was obtained in purified form from liver and was mixed with glutamic acid, ATP, and the indicated form of foiate. The mixture was incubated to permit the enzyme to catalyze the attachment of glutamafc residues to the folate. What do you think would be the effect of a vitamin Bij deficiency on the rate of polyglutamation of dietary folates, flint Dietary folates occur in the bloodstream initially as 5-melhyl-l UPteGtu, and enter the cell in this form. 

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