Glutamate diethyl ester

Spencer HJ (1976) Antagonism of cortical excitation of striatal neurons by glutamic acid diethyl ester evidence for glutamic acid as an excitatory transmitter in the rat striatum. Brain Res /02 91-101. 

Aso, K., Uemura, T., and Shiokawa, Y. (1988) Protease-catalyzed synthesis of oligo-L-glutamic acid from L-glutamic acid diethyl ester. Agric. Biol. Chem., 52... 

Uyama, H., Fukuoka, T., Komatsu, I., Watanabe, T., and Kobayashi, S. (2002) Protease-catalyzed regioselective polymerization and copolymerization of glutamic acid diethyl ester. Biomacromolecules, 3 (2), 318-323. 

Li, G Vaidya, A., Xie, W Gao, W and Gross, R.A. (2008) Enzyme-catalyzed oligopeptide synthesis Rapid regioselective oligomerization of L-glutamic acid diethyl ester catalyzed by papain. ACS Symp. Ser., 999,... 

Enzyme-Catalyzed Oligopeptide Synthesis Rapid Regioselective Oligomerization of L-Glutamic Acid Diethyl Ester Catalyzed by Papain... 

L-glutamic acid diethyl ester hydrochloride (600 mg, 2.5 mmol), papain (100 mg of water-soluble powder containing 40 mg protein), and 5 mL phosphate buffer solution set at a predetermined pH were transferred to a 15 mL Erlenmeyer flask. The flask was gently stirred in a water bath at 40 C for a predetermined reaction time. Then, the reaction mixture was cooled to room temperature and deionized water (20 mL) was added. The insoluble product was separated by centrifugation (6000 rpm), washed once with dilute HCl (2 % v/v)... 

Aso et al and Uyama et al found that crude papain extracts catalyzed oligomerizations of L-glutamic acid diethyl ester (conditions pH 7-8.5, 0.5M, ionic strength 2.0 M phosphate buffer, 25 C and pH 7.0, 40°C, 3 hours) giving oligomers with DP 5 to 9 and DPjvg 9.5 from FAB-Mass spectroscopy and H-... 

Figure 2. MALDI-TOF spectrum of oligo(y-ethyl L-glutamate) synthesized using 0.5ML-glutamic acid diethyl ester hydrochloride, 8 mg/mL catalyst, at 40 for 20 min, pH 7.0 under non-controlledpH conditions.

---