Glucosiduronic phenolphthalein

Glucuronidase Activity of Mammalian and Non-mammalian Tissues a. Phenolphthalein Liberated from Phenolphthalein p-o-Glucosiduronic Acid... 

Effect of Varying the Concentration of a Water Homogenate of Normal Adult Mouse Liver, Buffered with Acetate, During the Incubation with Substrate (0.00126 M Phenolphthalein fl-v-Glucosiduronic Acid).33 Homogenate Fractionated Before and After Incubation at 88°, and then Assayed... 

Fig. 1.—Fractionation33 of Water Homogenates of Mouse Liver at 1500 g after Varying Periods of Incubation at 38° in Acetate or Citrate Buffer, pH 5.2. (Homogenate and fractions assayed in the usual way with 0.00125 M phenolphthalein /3-d-glucosiduronic acid in the same buffer (a), normal adult liver (6), liver regenerating...

Fig. 2.—Hydrolysis of 0.00125 M Phenolphthalein /8-D-Glucosiduronic acid by Partially Purified Mouse-liver /3-Glucuronidase at Various pHs (in 0.125 N acetate (X), 0.125 N citrate ( ), and 0.125 N phthalate (O) buffer).

Dissociation Constants (Kx) for Various ff-o-Glucosiduronic Acids Treated as Competing Substrates in the Hydrolysis of Phenolphthalein fi-o-Glucosiduronic Acid by Mammalian and Non-mammalian fi-Glucuronidase... 

The experiments were carried out with partially-purified mouse-liver enzyme and phenolphthalein /3-D-glucosiduronic acid, in acetate buffer, pH 5.2, and dissociation constants were calculated by the method of Lineweaver and Burk.173... 

Boiled galactarate phenolphthalein /S-D-glucosiduronic acid 6.1 no inhibition 132... 

Fig. 4.—Hydrolysis48 of 6.3 X 10-4 M Phenolphthalein 0-D-Glucosiduronic acid at Various pH Values, in 0.05 N Acetate Buffer, by a Highly Purified 0-Glucuronidase Preparation from Female-rat Preputial Gland (Section IV), Alone (X), and in Presence of 0.03 % of Deoxyribonucleic Acid (O), or of 0.01% of Albumin ( ).

Various methods of purifying -glucuronidase from many materials have been reported. To compare the different i)roducts, it is most convenient to express the specific activities as glucuronidase unit/mg of protein, where 1 unit liberates 1 jig of phenolphthalein from phenolphthalein glucosiduronic acid in 1 hour at 38 C. The.se method.s and procedures, purities of enzymes and enzyme sources are summarized in Table TV. 

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