Glucosidases specificity

En me Treatment of LCCs. Purified Bacillus circulans xylanase (specific activity, 110 units/mg protein), purified Coriolus versicolor )9-mannanase (specific activity 179 units/mg protein), purified Thermoascus aurianticum /8-glucosidase (specific activity, 150 units/mg protein), and purified Schizophyllum commune acetyl xylan esterase (AXE specific activity, 180 units/mg protein) were used. Unit activity for xylanase and )9-mannanase was defined as the amount of enzyme catalyzing the release of 1 /xmol reducing power per minute at 50°C and pH 6.0 using oat spelts... 

Oikawa, A., Ebisui, K., Sue, M., Ishihara, A., and Iwamura, H. 1999. Purification and characterization of a p-glucosidase specific for 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) glucoside in maize. Z. Naturforsch. C Biosci. 54, 181-185... 

WARZECHA, H GERASIMENKO, I., KUTCHAN, T.M., STOCKIGT, J., Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis. Phytochemistry, 2000,54,657-666. 

It can be seen from Table 2 that a large number of the naturally-occurring polyhydroxylated alkaloids are potent inhibitors of the various a-glucosidase-specific disaccharidases involved in mammalian digestion (e.g. sucrase, maltase, isomaltase, etc.,). These enzymes are expressed at the surface of the epithelial cells of the brush border in the small intestine. In the late 1970s it was realised that inhibitors of these enzymes, such as DNJ, could be used therapeutically in the oral treatment of the non-insulin-dependent (type n) diabetes mellitus. ... 

Purification step and fraction Total protein (mg) Total activity (unit) cellulase 3-glucosidase Specific activity (unit/mg) cellulase 3-glucosidase ... 

In the area of simple terpene glycoside farnesyl fi-D-galactopyranoside and a- and -D-mannopyranoside have been reported as have eighteen monoterpene j8-D-glucopyranosides for an investigation of /S-glucosidase specificity. The soluble silver trifluoroacetate gave appreciably enhanced yields. ... 

Acarbose and Miglitol These agents are specific inhibitors of intestinal glucosidases and reduce the conversion of sucrose and starch to glucose. Their main effect is a delay, not a complete inhibition, of the absorption of carbohydrates. Postprandial blood sugar excursions are effectively reduced. Because a small portion of the carbohydrates enters the colon, their microbial degradation frequently causes flatulence or... 

The question of a correlation of anomeric specificity of glycosidases with their susceptibility to aldonolactone inhibition was addressed by Reese and coworkers in a comparative study with six fungal a-D-glucosidases and... 

Shulman and coworkers showed that ) -D-glucosyl isothiocyanate (43) can be used for the specific inactivation of ) -D-glucosidase B from sweet... 

Figures 1 and 2 show positive correlation, although of varying degrees of goodness of fit, between the specific activities of endo ucanase, exoglucanase, and -glucosidase activities with the cellulose composition and degradation in digester feed, respectively. These data indicate a direct relationship between the cellulose content in the feed and cellulase enzyme complex production by the digester consortia. In this system, as in many others studied, cellulose is an effective inducer of cellulase secretion (67,68),...

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