8-Glucosidases purification

The only other examples of bromoconduritol inhibition reported so far are a cytosolic jff-D-glucosidase from calf liver and the lysosomal ff-D-glu-cosidase from calf spleen. In spite of the 6500-fold difference in their reactivity with conduritol B epoxide (see Table XI), both enzymes are rapidly inactivated by bromoconduritol F, with kj(max)/Kj 10 M min for the cytosolic enzyme and lq(max)/Ki 3.2 10 for the crude and 3.9 10 M min for the purified lysosomal enzyme. It should be noted that purification of the lysosomal jS-D-glucosidase had effects on the reactivity with bromoconduritol F similar to those it had on the reactivity with conduritol B epoxide (see Table XI). 

Neculai MA et al (2009) Partial purification and characterization of three ginsenoside-metabolizing b-glucosidases from Pythium irregulare. Phytochemistry 70 1948... 

Zhang C et al (2001) Purification and characterization of a ginsenoside-f -glucosidase from ginseng. Chem Pharm Bull 49 795... 

Shin HY et al (2003) Purification and characterization of a a-L-arabinopyranosidase and a-L-arabinofuranosidase from Bifidobacterium breve K-110, a human intestinal anaerobic bacterium metabolizing ginsenoside Rb2 and Rc. Appl Environ Microbiol 69 7116 Park SY et al (2001) Purification and characterization of ginsenoside Rbi-metabohzing P-glucosidase from Fusobacterium K-60, a human intestinal anaerobic bacterium. Biosci Biotechnol Biochem 65(5) 1163... 

Yan Q et al (2008) Purification method improvement and characterization of a novel ginsenoside-hydrolyzing-glucosidase from Paecilomyces bainier sp. 229. Biosci Biotechnol Biochem 72 352... 

K. M. Osiecki-Newman, D. Fabbro, T. Dinur, S. Boas, S. Gatt, G. Legler, R. J. Desnick, and G. A. Grabowski, Human acid p-glucosidase Affinity purification of the normal placental and Gaucher disease splenic enzymes on N-alkyl-deoxynojirimycin-sepharose, Enzyme, 35 (1986) 147-153. 

G. Legler and L. M. O. Osama, Mechanism of action of glycosidase splitting enzymes. IV. Purification and properties of a P-glucosidase from Aspergillus oryzae, Hoppe Seyler s Zeitschr. Physiol. Chem., 349 (1968) 1488-1492. 

T. B. Frandsen, F. Lok, E. Mirgorodskaya, P. Roepstorff, and B. Svensson, Purification, enzymatic characterisation and nucleotide sequence of a high-isoelectric-point a-glucosidase from barley malt, Plant Physiol., 123 (2000) 275-286. 

A. Hentges and E. Bause, Affinity purification and characterization of glucosidase II from pig liver, Biol. Chem., 378 (1997) 1031-1038. 

Thus a biospecific adsorbant was obtained selective desorption should therefore permit successive elution of cellulolytic enzymes. It was thus found that the method was very useful in the purification of the cellobio-hydrolases from Tr. r., starting from very crude culture filtrates. Addition of glucose (0.1M) or gluconolactone suppressed the action of glucosidases present, preventing deterioration of the columns. The capacities exceeded 10 mg CBH I per ml gel (prepared with CNBr activated Sepharose). 

Le Traon-Masson, M.-P. and Pellerin, P., Purification and characterization of two P-D-glucosidases from an Aspergillus niger enzyme preparation affinity and specificity toward glucosylated compounds characteristic of the processing of fruits. Enzyme Microb. Technol 22, 374, 1998. 

Odoux E, Chauwin A, Brillouet JM (2003) Purification and characterization of vanilla bean (Vanillaplanifolia Andrews) / -glucosidase. J Agric Food Chem 51 3168-3173 Overhage J, Steinbuechel A and Priefert H (2003) Highly efficient biotransformation of euge-nol to ferulic acid and further conversion to vanillin in recombinant strains of Escherichia coli. Appl Environ Microbiol 69 6569-6576... 

Ranjoanisafy X (1998) Purification et etude de quelques proprietes de la jS-glucosidase de la vanilla. Implications dans la preparation de la vanilla. Master s thesis, Ensia-Siarc, Montpellier Rao SR and Ravishankar GA (2000) Vanilla flavour Production by conventional and biotechnological routes. J Sci Food Agric 80 289-304... 

The sequential and cooperative roles of the enzymes are shown in Figure 1. Endoglucanases, often called cellulases or carboxymethylcellu-lases, provide nonreducing chain ends from which the exoCBH s cleave cellobiosyl residues. The cellobiose, which is inhibitory to the depoly-merizing enzymes, is cleaved to glucose by the / -glucosidase. Since the product of one enzyme may become the substrate for another, enzyme purification is an essential prerequisite to any clear description of the system. 

Crombie, H.J., Chengappa, S., Hellyer, A. and Reid, J.S.G. (1998). A xyloglucan oligosaccharide-active, transglycosylating beta-D-glucosidase from the cotyledons of nasturtium (Tropaeolum majus L) seedlings — purification, properties and characterization of a cDNA clone. Plant J., 15, 27-38. 

Oikawa, A., Ebisui, K., Sue, M., Ishihara, A., and Iwamura, H. 1999. Purification and characterization of a p-glucosidase specific for 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) glucoside in maize. Z. Naturforsch. C Biosci. 54, 181-185... 

Odoux, E., Chauwin, A. and Brillouet, J.M. (2003) Purification and characterization of vanilla bean (Vanilla planifolia Andrews) beta-D-glucosidase. Journal of Agricultural and Food Chemistry 51 (10), 3168-3173. 

LUIJENDIJK, T.J.C., STEVENS, L.H., VERPOORTE, R., Purification and characterization of strictosidine (3-D-glucosidase from Catharanthus roseus cell suspension cultures. Plant Physiol. Biochem., 1998,36,419-425. 

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