Glucose with glutamic acid

The design of the pyrolysis reactor was such to convert all of the organic material, either suspended or dissolved, in a 50- t1 wastewater sample, to volatile organic compounds that would then be measured as a single peak by the FID. A standard mixture of glucose and glutamic acid was pyrolyzed to calibrate the unit. By entering the BOD value of the standard mixture into the final calculation, the results obtained by Py-FID could be directly compared with the BOD values for the same samples. 

If cells are.saturated with glutamic acid by incubation in the presence of that amino acid and glucose, then washed and resuspended in buffered saline at pH 7.0, no significant loss occurs from Strep, faecalis, whereas a... 

Fig. 10. Progress curves for the accumulation of glutamic acid within washed suspensions of Staph, aureus incubated with glutamic acid and 0.04% glucose under...

There is no evidence that crystal violet will inhibit the internal metabolism of glutamic acid in Staph, aureus as it does in Strep, faecalis. If the amount of glutamic acid in the cells and external medium is determined before and after incubation of the cells with glutamic acid, there is an overall loss of glutamic acid on balance, but the extent of this loss appears to depend to a large degree upon the amount of glucose present in the incubation mixture. Table VI shows that if small amounts only of... 

Suspensions of deficient Staph, aureua were incubated with glucose and glutamic acid with and without the addition of one other amino acid at a concentration equal to twice that of the glutamic acid. The rate of glutamic acid accumulation was determined in each case and the effect of the second amino acid expressed as per cent of the rate obtained with glutamic acid as the only amino acid present... 

Terner, Eggl ton, and Krebs (1950) found that brain cells incubated with glucose or glutamic acid alone lost potassium ions, whereas when incubation took place in the presence of both glucose and glutamic acid, the potassium was retained. The amount of potassium retained under these conditions was approximately equivalent to the glutamic acid... 

Fig. 16. Accumulation of free glutamic acid within Staph, aureus incubated with curve 1, diethyl glutamic ester 2, iV-phosphorylglutamic acid 3, as 2 with a second addition of A -phosphorylglutamic acid at time indicated by arrow 4, IV-phosphoryl-glutamic acid and glucose 5, glutamic acid and glucose. Sources added to the external medium at concentrations sufficient to saturate the accumulation mechanism in all cases falling of rate in curve 2 is due to decomposition of the iV-phosphorylglutamic acid.

Organisms incubated with glucose and glutamic acid with and without a complete mixture of the other natural amino acids. Final concentration of amino acids, other than glutamic acid, = 0.16 mg./ml. = 1 on scale below suspension density of organisms during incubation = 2.0 mg. dry weight of cells/ml. 

Fig. 19. Effect of internal concentration of free glutamic acid on the rate of protein synthesis (measured by the increase in combined glutamic acid of the cells) in Staph, aureus. Broken lines indicate internal concentration of free glutamic acid continuous lines, protein synthesis open points, cells pretreated with glucose and glutamic acid solid points, cells pretreated with glucose only. (Gale, 1951b.)...

A modification of (1) the surface structures of the cell are impermeable to glutamic acid but there exists within the cell wall itself a carrier substance which, in the presence of glucose, will combine with glutamic acid and transport it across the barrier. This type of hypothesis has been discussed by Osterhout (1952) in another connection. 

Synonyms Glutamic acid, reaction prods, with glucose Definition Ester of glucose and glutamic acid Empirical C H jNOj... 

Another problem is the cahbration of BOD-sensor to enable the comparison with the conventional BOD. For cahbrating a BOD-sensor, a glucose-glutamic acid standard solution (the so-called GGA-standard), which is also used as standard solution for the BODj-method [71], or aqueous solutions of glucose or glycerol with a defined BODj [43,54] are used. 

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