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Glucose sensitive electrodes

A D-glucose-sensitive electrode has been constructed by coating of a platinum electpode with a cross-linked poly(vinylalcohol) layer containing immobilised D-glucose oxidase and ferrocene. ... [Pg.8]

Figure 4.28 Calibration curves of a glucose-sensitive electrode, based on the PO2 transducer, as a function of oxygen partial pressure in the sample, between 100 and 800 mm Hg. Figure 4.28 Calibration curves of a glucose-sensitive electrode, based on the PO2 transducer, as a function of oxygen partial pressure in the sample, between 100 and 800 mm Hg.
As the solution passes through the column, the glucose is enzymatically oxidized to 8-D-gluconolactone (which is hydrolyzed to gluconic acid nonenzymatically) with utilization of O2. Change in O2 concentration, related to the amount of glucose in solution, is determined with 02-sensitive electrodes placed at the top and bottom of the column. The column may be used repeatedly. [Pg.36]

Techniques such as potentiometry, polarography, and microcalorimetry have been chosen in exploiting the benefits of immobilized enzymes (see Chapter 4). Enzymes incorporated into membranes form part of enzyme electrodes. The surface of an ion-sensitive electrode is coated with a layer of porous gel in which an enzyme has been polymerized. When the electrode is immersed in a solution of the appropriate substrate, the action of the enzyme produces ions to which the electrode is sensitive. For example, an oxygen electrode coated with a layer containing glucose oxidase can be used to determine glucose by the amount of oxygen consumed m the reaction, and urea can be estimated by the combination of a selective ammonium ion-sensitive electrode and a urease membrane. [Pg.213]

Both the sensitivity and the specificity of a glucose microenzyme electrode have been improved by using voltage pulses (Ikariyama et al., 1988). [Pg.30]

Several authors investigated ion selective electrodes incorporating GOD coupled with oxidative reactions catalyzed by horseradish peroxidase (HRP). Thus, glucose has been determined by measuring iodide concentration at an iodide sensitive electrode (Nagy at al., 1973) according to ... [Pg.92]

As an alternative to the catalytic activity of a conjugated enzyme, the complement-mediated immunolysis of liposomes can be used to amplify the measuring signals of immunoassays. The liberation of liposome-entrapped markers depends on the amount of antibody adhering to the lipid membrane and serves to measure the concentration of antigen. Such liposomes may be filled with quarternary ammonium ions detectable by ion sensitive electrodes (Umezawa et al., 1983), with enzyme substrates, e.g. glucose, which can be measured with enzyme electrodes... [Pg.270]

Glycoenzyme immobilization via their carbohydrate moieties enables bonds to be formed outside the enzyme active site and thus the enzyme retains its original activity. The new technique described above for the preparation of an enzyme electrode was used primarily for the immobilization of glucose oxidase to prepare a D-glucose sensitive sensor. The enzyme electrode kept 95% of its original activity after 2 months during which about 900 analyses were performed. The detection limit of D-glucose was 17 jumol/1. [Pg.403]


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