Glucose pulse

Miller WM, Wilke CR, Blanch ITW (1989), Transient response of hybridoma cells to nutrient additions in continuous culture I. Glucose pulse and step changes, Biotechnol. Bioeng. 33 487-499. 

Fig. 6.4 Temporal behavior of the normalized signal S when a glucose pulse is applied, increasing the glucose from 5 mM to 10 mM and lasting for 60 min. The PI control features are clearly seen, starting with a rapid proportional response, followed by a slow integral control. Increasing the time constant k-, responsible for the integral control, results in a faster saturation of the response. The smallest and maximal value of the active fraction is Xq = 0.2 and Xmax = 0-8 respectively.

Figure 25-13 Response of plasma insulin to glucose stimulation. A 20-g glucose pulse is given intravenously at time 0. A, Healthy subjects. B, Patients with type 2 diabetes mellitus (NIDDM). C, Patients with type I diabetes mellitus (IDDM). IRf, Immunoreactive insulin. Values before time 0 represent baseline. (From Pfeifer MA, Halter JB, Porte D Jr. Insulin secretion in diabetes mellitus. Am J Med 1981 70.-579-88.)...

Figure 8. Experimental curves obtained after injection of ImM glucose pulses (in O.IM potassium phosphate pH 7.0) of varying durations. Arrows indicate substrate introduction CIS).

In 7 healthy subjects pretreatment with oral theophylline for at least 4 days significantly increased the fall in serum potassium levels and rises in blood glucose, pulse rate, and systolic blood pressure caused by an infusion of terbutaline. A study in children given slow-release formulations of both theophylline and terbutaline found no increases in reported adverse effects and simple additive effects on the control of their asthma. ... 

The turnover rates of glycine in different regions have also been calculated from the assumption that the [ J-amino acids disappear from one open pool at steady-state conditions (Freeman et al., 1983). The decrement in radioactivities at 60 and 90 min postinjection of the glucose pulse has been employed to calculate the half-life. 

Fig. 24. Transient glucose concentration in response to a glucose pulse during continuous cultivation of Escherichia coli (D = 0.1 h" )...

Fig. 27. Concentration distributions of glucose, ATP, and pyruvate four seconds after a glucose pulse onto the liquid surface...

Mashego, M.R., van Gulik, W.M. et al (2009) Fast dynamic response of the fermentative metabolism of Escherichia coli to aerobic and anaerobic glucose pulses. Biotechnol. Bioeng, 104 (6), 1153-1161. 

Sunya, S., Gorret, N., Delvigne, E, Uribelarrea, J.L. et al (2012) Realtime monitoring of metabolic shift and transcriptional induction of yciG-.-.luxCDABE E. coli reporter strain to a glucose pulse of different concentrations. J. Biotechnol, 157 (3), 379-390. 

C., and Gorret, N. (2013) Short-term dynamic behavior of Escherichia coli in response to successive glucose pulses on glucose-limited chemostat cultures. 

Figure 32 Heat flow rate during repeated glucose pulses to a culture of S. cere-visiae. (Reproduced from Reference [14] with permission of the author and publisher).

Figure 37 Heat flow rate and total heat released during controlled fed-batch culture of Saccharopolyspora erythraea by pulsing glucose after ammonium depletion of the culture shown in Figure 36. The depletion of glucose was easily detected by the sharp heat flow rate decrease immediately followed by the injection of a glucose pulse. (Redrawn from Reference [81] with permission of the author and publisher).

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