Glucose-6-phosphatase expression

Nakae, J., Kitamura, T., Silver, D. L., and Accili, D. (2001) The forkhead transcription factor FoxOl (Fkhr) confers insulin sensitivity onto glucose-6-phosphatase expression. 

Gluconeogenesis 1 TORC2, others l Expression PEP carboxykinase, glucose-6-phosphatase Liver... 

Argaud, D., Kirby, T.L., Newgard, C.B. and Lange, A.J. (1997) Stimulation of glucose6-phosphatase gene expression by glucose and fructose-2, b-bisphosphate. J. Biol. Chem., 272, 12854-12861. 

Massillon, D., Barzilai, N., Chen, W., Elu, M. and Rossetti, L. (1996) Glucose regulates in vivo glucose-6-phosphatase gene expression in the liver of diabetic rats.J. Biol. Chem., 271, 9871-9874. 

Chen, R., Meseck, M., McEvoy, R. C. and Woo, S. L. (2000). Glucose-stimulated and self-limiting insulin production by glucose 6-phosphatase promoter driven insulin expression in hepatoma cells. Gene Ther. 7, 1802-1809. 

Lochhead, P. A., Salt, I. P., Walker, K. S., Hardie, D. G., and Sutherland, C. 2000. 5-aminoimidazole-4-carboxamide riboside mimics the effects of insulin on the expression of the 2 key gluconeogenic genes PEPCK and glucose-6-phosphatase. Diabetes 49 896-903. 

Proximal tubule cells in culture should have retained functional attributes such as (1) polar architecture and junctional assembly of epithelia and correct membrane distribution of enzymes and transport systems (2) vectorial transport of solutes and water, manifested by the formation of domes when cultured on solid supports [81] and the generation of transepithelial electrophysiological properties [82, 83] due to the expression of proximal tubule specific claudins 2- and 10 [84, 85] (3) cellular uptake of xenobiotics from either the apical or basolateral side, as observed in vivo and (4) expression of nephron segment-specific characteristics, i.e., distinct expression of differentiation markers, metabolic and transport properties, and hormone responsiveness. Such markers include the expression of the brush border enzymes alkaline phosphatase, leucine aminopeptidase, and y-glutamyl transferase [4, 86], In addition, proximal tubule cells should possess Na+,K+-ATPase activities, Na+-dependent glucose, and p-aminohippurate transport. Proximal tubule cells increase cAMP levels in response to parathyroid... 

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