1,3-Glucans, oligomers

Figure 1 Separation of fi-glucan cellodextrin- - )/i-D-glucose oligomers by HPAEC. (-4) Detection by PAD of authentic oligomers from p-glucan added to the reaction product as cm elution marker. (B) Determination of radioactivity in fi-glucan oligomers synthesized in vitro...

Pang Z., Otaka K., Maoka X, Hidaka K., Ishijima S., Oda M., Ohnishi M., Structure of beta-glucan oligomer from laminarin and its effect on human monocytes to inhibit the proliferation of receptor binding in the CR3- human promonocytic cell line U937, Biosci. Biotechnol. Biochem., 69(3), 2005, 553-558. 

Miyanishi, N., Iwamoto, Y., Watanabe, E. Oda, T. J. (2003). Induction of TNF-a production from human peripheral blood monocytes with p i,3-glucan oligomer prepared from laminarin with pi,3-glucanase from Bacillus clausii NM-1. Journal of Bioscience and Bioengineering, 95, 192-195. 

Pang, Z., Otaka, K., Maoka, T., Hldaka, K., Isljlma, S., Oda, M. Ohnlshl, M. (2005). Structure of P-glucan oligomer from lamlnarln and Its effect on human monocytes to Inhibit the proliferation of U937 cells. Bioscience and Biotechnology Biochemistry, 69, 553-558. 

Vacuum-ultraviolet, circular dichroism (v.u.c.d.) measurements have been made on films and solutions of linear D-glucan in a study of the conformation of dextran and its oligomers.123 Film formation of the linear dextran was accompanied by crystallization, and the v.u.c.d. band was observed at 165 nm, in contrast to nonlinear dextran films displaying a band at 177 nm. The difference was ascribed to hydrogen bonding of the ring-oxygen atom in the crystalline state. 

As the X-ray diffraction diagrams of an oriented gel (Fig. 6) are poorly crystalline, it has been useful to conduct X-ray crystal structure analyses of oligomer of 3-(l->-3)-D-glucan. The molecular structures of laminarabiose and its acetyl derivative have been determined. 

The most widely distributed polysaccharide on earth is cellulose, which is built up in plants as a skeletal compound. Cellulose is depolymerized by acetol-ysis, that is, by treatment with a mixture of acetic acid anhydride and sulfuric acid. The y0-glucopyranose residues in cellulose are linked in 1-4-position. Figure 3.247 shows the chromatogram of an aqueous solution of -(l-4)-linked D-glucans, which was obtained by Koizumi et al. [236] after partial acetolysis of cellulose and subsequent deacetylation in 0.15 mol/L NaOH. Oligomers up to DP28 were detected. 

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