Glucanases between

Synergism between l,3-/9-Glucanases in Yeast Cell Wall Zymolysis... 

Table L Synergism between Glucanases in C wriffis lysis (SCL) and Solubilization of Cell Wall Glucans (SCS)...

Synergism between the enzymes was shown to decrease with time during zymolysis. This may occur because the substrate for a particular glu-canase disappeared (but as the substrates were in excess in this case it is unlikely), or the products released during hydrolysis inhibited one or a number of the glucanases. 

Varghese and coworkers have identified the active site nucleophiles of two crystalline ) -D-glucan endohydrolases from germinating barley [70]. In more recent work, Hej and coworkers were able to introduce 2,3-epoxypropyl- -laminaribioside 8 (n = 1) into the active site of the crystalline l,3-/3-D-glucanase from barley [71]. A subsequent X-ray crystallographic investigation gave invaluable information about the active site of this enzyme and the point of attachment between enzyme and inhibitor was confirmed. 

This study describes an absolute method for the evaluation of endo-/ -glucanase (Cx) or endocellulase activities in the cellulase complex without the need of a time-consuming isolation of the endocellulase fractions. In the method proposed by Almin et al. (3), an assumption was needed in the theoretical derivation of endocellulase activities, namely, that Mv/Mn is constant in the initial stages of the enzymic reactions. We found, however, a linear relationship between Mv and STn. They used CMC as substrate, and since the mode of action of endocellu-lases on CMC can be regarded as similar to that on HEC, it is possible that their assumption is not fulfilled. 

Test Preparation Prepare a solution from the enzyme preparation sample so that 1 mL of the final dilution will contain between 0.01 and 0.02 (3-glucanase units. Weigh the sample, transfer it into a volumetric flask of appropriate size, dilute to volume with Phosphate Buffer, and mix. 

The ratio of lytic protease to glucanase in the lytic system The effect of pH or temperature on synergism between the... 

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