Glass-slide holder

Figure 13, Hexagonal theta specimen fracture illustrating the common fragments, (a) shows the broken pieces seen through the glass slide holder and (b) shows them after extraction.

Embryo sections 1-11 are incubated at 40°C for overnight. Sections 1-10 on Si chips are then placed between clean glass slides that are pre-loaded in a glass slide holder (tee Note 4). 

Cut 4-pjn-thick sections with a microtome and float them on a water bath kept at room temperature so as to stretch the sections. An ordinary glass slide is used to transfer the sections onto another water bath kept at 58°C to further stretch the sections. Lift them by the SuperFrost slides, thus mounting them in the process. The sections are allowed to dry in an upright position in a slide holder at a temperature of <30°C. When the slide holder is full, it is transferred to a conventional oven. 

The micro channel system was fabricated by standard silicon micromachining via etching of a silicon wafer with potassium hydroxide using thermal oxide as an etch mask [6], The double mixing tee configuration consists of six micro channels. For fluid connection, an outlet hole was drilled into the silicon chip. The chip was anodically bonded to a glass slide with three inlet holes, clamped in a holder and, thereby, connected to a commercially available quench-flow instrument... 

The time-dependent particles were collected on membrane filters (47 mm, 0.1 and 1.0 /xm, Nucleopore Corp.) contained in polycarbonate filter holders (Nucleopore Corp.). The filter holders were also cleaned in a similar manner as the glass slides to prevent cross contamination from previous sampling. As with the impactor studies, the sampling train included a low-volume pump, flow meter, and manometer. This enabled accurate measurements of the flow-rate of ambient air to be made... 

Figure 3.22. Excess powder is removed from the top of the sample holder with a single sweep by an edge of a razor blade or a glass slide. The direction of the sweep depends on the physical properties of the powder and is usually established by trial-and-error.

True random orientation mounts are difficult to prepare, and numerous techniques for filling powder sample holders have been described (Brindley and Brown, 1980). For example, powder samples have been embedded in plastic (Brindley and Kurtossy, 1961), polyester foam (Thompson et al., 1972), mixed with powdered cork (Wilson, 1987), pasted in acetone prior to smearing onto glass slides (Paterson et al., 1986) and spray dried (Hillier, 2002). 

Thin films of a blend of poly(sebaic acid) and PLA (Polysciences) on glass are prepared by spin-coating from 1 w/v % solutions in chloroform onto cleaned glass slides. The glass slides are glued with epoxy to the metallic sample holder disc. 

In order to perform the adhesion tests, the prepared samples were mounted in position in the heating chamber as shown in Fig. 23.3. The elastomer side of the sample was previously attached to a glass slide using a silicone glue and the slide was fastened into place on the sample holder, which was then coimected... 

Slide/cover slip washing containers For slides, it is convenient to perform washes in either Wheaton slide-staining jars with removable slide holders (Thomas Scientific) or Coplin jars (Thomas Scientific). For Coplin jars, each wash is 50-70 ml staining jars require about 200 ml to cover the slides. For samples on cover slips, use glass cover slip-staining jars (Thomas-Scientific) each wash is 8-10 ml. 

With a slide holder designed for use with the Minimet , the mounted material is ground by hand with 5.0- im alumina on a glass plate. Isopropyl alcohol or polishing oil is used as the vehicle. 

Grinder-Polishers with extra wheels and Minimet (Buehler), the latter with slide holder for thin sections 2 Glass plates (300x300 mm) Vacuum impregnator Epoxy and hardener and polyethylene cups (several varieties of each) Cyanoacrylate adhesive (Super Glue )... 

In the DSC thermal methods, the samples were isothermally annealed in the sample holder at various temperatures for 4-5 min and then quickly quenched to room temperature before increasing the temperature at a scanning rate of 20°C/min in the DSC-7 to look at glass transition behavior. In the optical methods, films were converted with a glass slide and annealed on a hot stage at various constant temperatures around the phase separation temperature for a certain period of time. The phase separation temperature was taken as the lowest annealing temperature at which the film was found to be cloudy. 

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