Slide holders

Position slides in slide holders with filter cards and sample chambers and attach to cytocentrifuge rotor. 

Place the slides requiring microwave irradiation in plastic slide holders in the citric acid solution. 

Metallic tools should never be used in a MWO therefore, heat-resistant plastic Coplin jars or containers and heat-resistant plastic slide holders with a capacity of at least 15 sections are required for this application. MWO-medi-ated HIER can also be performed in a plastic pressure cooker designed for household kitchen application (see Subheading 3.2.), which appears to eliminate some of the drawbacks of the standard MWO protocols. 

Clean staining dishes, forceps, and slide holders wrap them with an aluminum foil, and bake them at 350-400°C for approx 4-6 h. 

At the end of the 1-h heat treatment, carefnlly transfer all slides from the oven, and place them in a slide holder. 

Wash slides twice (5 min each) by gently moving the slide holder up and down in 3X PBS. 

Place a clean sheet of aluminum foil on a bench top work area. Carefully remove each slide from the slide holder. Wipe remaining alcohol around the periphery of the tissue with Kim-wipe and place it on the clean sheet of aluminum foil. 

Place them in a slide holder and treat with DEPC-treated IX PBS for 5 min. Dehydrate in graded alcohol 50, 70, 95, and 100% for 2 min each. 

The Code-On is a capillary gap Stainer. The capillary gap is formed between two slides, each of which may bear specimens. The shdes of a pair are kept approx 150 tim apart by the clamping action of the slide holder, and by raised areas that exist on each slide at the label area and at each of the opposite corners at the end of the slide. The capillary gap, enclosed by the two slides, holds approx 150 tiL of reagent, assuming that the gap fills to a height of approx 1.5 in. from the bottom of the slide pair. 

To assess the effect of sloppy loading of slides into the platen of the Ventana, a series of bar-coded slides were placed into the slide holders on the platen at a variety of angles or placed on the instrument unclipped into the slide holders. In all cases, when the instrument scanned the slides, it reported error conditions and would not allow the staining run to proceed. 

Cut 4-pjn-thick sections with a microtome and float them on a water bath kept at room temperature so as to stretch the sections. An ordinary glass slide is used to transfer the sections onto another water bath kept at 58°C to further stretch the sections. Lift them by the SuperFrost slides, thus mounting them in the process. The sections are allowed to dry in an upright position in a slide holder at a temperature of <30°C. When the slide holder is full, it is transferred to a conventional oven. 

Substrate = 3f>l SLIDE (8-PINS/7-FIELDS) Holder = SLIDE HOLDER ... 

Fill incubation container with enough retrieval solution (200 mL) to cover slides and insert slide holder. Insert slides in holder and cover. 

Place the slides receiving microwave irradiation in plastic slide holders in the citric acid solution. These solutions are also commercially available (Dako Inc., Carpinteria, CA Biogenex Corporation, San Ramon, CA or Biocare Medical, Concord, CA) (rrrNote 5). 

Lift open the top cover, and you will see a brass slide holder. This moves on a track and is removable. 

The most common device for displaying tagboard placards, particularly on tank cars, is a slide holder. The holder is manufactured of either aluminum or heavy duty steel, and permits the insertion and removal of a tagboard placard. More recently, a 20 mil rigid vinyl has been introduced which is far more durable than tagboard and may be reused for dozens of trips. 

One of the most popular substitutes for tagboard has been a 4 mil flexible vinyl with a removable pressure sensitive adhesive. This material has the virtue of complete exterior durability, yet it can be removed easily, when required, without leaving a residue. A slide holder is not required. Instead, these placards are affixed directly on the tank. 

A micro-adaptation of energy dispersive X-ray fluorescence (EDXRF) has been used to compare the Ni content of normal and carcinomatous human breast tissue (Rizk and Sky-Peck, 1984). Surgical specimens were homogenized (200 g/L in water) in a Teflon homogenizer. Ten 5 aliquots of each specimen were layered onto Formvar films in a slide holder, air-dried under laminar flow, and placed in the vacuum chamber of the instrument at 200 /im of mercury. By X-irradiation for 500 s (18 mA, 35 kV) 16 elements were determined simultaneously. The Ni scattered peak was observed at 7 keV and Ni concentrations of about 1 mg/kg dry wt. were caiculated after computer correction of the background continuum, as described (Sky-Peck and Joseph, 1981). 

Slide/cover slip washing containers For slides, it is convenient to perform washes in either Wheaton slide-staining jars with removable slide holders (Thomas Scientific) or Coplin jars (Thomas Scientific). For Coplin jars, each wash is 50-70 ml staining jars require about 200 ml to cover the slides. For samples on cover slips, use glass cover slip-staining jars (Thomas-Scientific) each wash is 8-10 ml. 

Fig. I. Apparatus used to make polarised fluorescence intensity measurements from the top (a), the cell compartment (bl, and from the side (c). Xenon arc (Xe), monochromator (M), lens (L). filter (Fi), polariser (Pi), outside shell (OS), inside shell (IS), ciaet (Cu), slide (S), mask (Ma), filter (F2K polariser (Pi), photo-cathode (PC). photomultiplier (PM), power supply (PS), microammeter (A), locking collar (C), scale (Sc), slide holder (H), hole in mask (Ho), movable support (Su).

With a slide holder designed for use with the Minimet , the mounted material is ground by hand with 5.0- im alumina on a glass plate. Isopropyl alcohol or polishing oil is used as the vehicle. 

After cleaning the slide, slide holder, and hands, the final polish is accomplished with the Minimet with 0.3- im alumina and polishing oil or propylene glycol on Texmet . 

Between polishing steps, the mount and slide holder should be cleaned with a forceful isopropyl alcohol spray. The hands may he washed with soap and water. Before examination, the polished section should be cleaned in a sonic cleaner with isopropyl alcohol, followed by a brief rinse with an alcohol spray. 

Grinder-Polishers with extra wheels and Minimet (Buehler), the latter with slide holder for thin sections 2 Glass plates (300x300 mm) Vacuum impregnator Epoxy and hardener and polyethylene cups (several varieties of each) Cyanoacrylate adhesive (Super Glue )... 

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