Genotypic detection method

Parsons, B. L., and Heflich, R. H. (1998). Detection of a mouse H-ras codon 61 mutation using a modified allele-specific competitive blocker PCR genotypic selection method. Mutagenesis 13, 581-588. 

Sequence-specific metihods are needed for the rapid detection of oligonucleotides for genotyping and diagnosis of infections and various genetic diseases. Most current detection methods use a fluorescent or eiectroactive tag bound to the analyte or to the probe (7-5). An assay that does not require any chemical manipulation (d, 7) of nucleic acids or complex reaction mbctures would... 

The genotypes to be detected by a genetic test must be shown by scientifically valid methods to be associated with the occurrence of a disease. The observations must be independently replicated and subject to peer review" (Holtzman and Watson, 1998, p. 25). 

Mycobacteria of the Mycobacterium avium complex are implicated in disseminated bacterial infections in AIDS patients. RFLP studies followed by hybridization with radiolabeled probe specific for an insertion sequence in M. avium (IS 1311) have been useful for typing M. avium stains (R2). A variety of molecular techniques are available for the diagnosis of Chlamydia trachomatis infection. In addition to PCR, a method based on the ligase chain reaction has also been found to be sensitive to the detection of C. trachomatis infection in urine specimens collected from male and female subjects (VI). The differentiation between low-risk genotypes of human papilloma virus (HPV 6 or 11) from genotypes of high... 

---