Genosensors oligonucleotides

M.I. Pividori, A. Merkoci and S. Alegret, Electrochemical genosensor design immobilisation of oligonucleotides onto transducer surfaces and detection methods, Biosens. Bioelectron., 15 (2000) 291-303. 

The ply genosensor has been used for detecting oligonucleotide sequences containing a one- or three-base mismatch. Three different... 

In the optimised experimental conditions the ply genosensor has been tested for different concentrations of the complementary oligonucleotide target. In the case of the enzymatic detection, a linear relationship between peak current and concentrations of complementary ply target has been obtained between 0.1 and 5 pg/pL, with a correlation coefficient of 0.9993, according to the following equation ... 

Thus, this review will focus on electrochemical genosensor developed using carbon screen-printed electrodes as the transducer the methods to immobilize oligonucleotides onto carbon based surfaces will be reviewed and special emphasis will be given to describe the electrochemical adsorptive accumulation. 

Also, intercalated adriamycin has been used as hybridization label in a genosensor built by modifying a glassy carbon electrode (GCE) with multiwalled carbon nanotubes with carboxyl groups and Au-NPs [23], Differential pulse voltammetry (DPV) was utilized to monitor the DNA hybridization event. Under the optimal conditions, the increase of reduction peak current of adriamycin was linear with the logarithm of the concentration of the complementary oligonucleotides from 1.0 x 10 to 5.0 x 10 M with a detection limit of 3.5 x 10 M. 

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