Genome promoter optimization

Reasonable access to appropriately patented genomic inventions should be provided for research purposes and exclusive positions maintained for commercial uses. This will promote rapid access to genomic information and fundamental technologies while optimizing the discovery and disclosure of basic advances in biomedical research. The primary question behind the decision whether to patent a finding from genomic research is Will patent protection for this invention promote its development For a potential therapeutic protein, the... 

For the integrating vector constructs we are using to express fluorescent proteins under control of the actin 15 promoter, there are two ways of modulating the expression level. First, to select an appropriate clone after transformation of the cells (for details see(6)). These vectors integrate randomly into the genome, and the level of expression depends on the site of integration. Second, even in a clonal population the expression varies from cell to cell. If green and red fluorescent proteins are expressed simultaneously, their levels fluctuate independently. Therefore, the experimentalist has to choose cells within a population that exhibit the optimal levels of expression (tee Note 4). 

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