Genome analysis alternative splice

Indik, Z., Yeh, H., Ornstein-Goldstein, N., Sheppard, P., Anderson, N., Rosenbloom, J. C., Peltonen, L., and Rosenbloom, J. (1987). Alternative splicing of human elastin mRNA indicated by sequence analysis of cloned genomic and complementary DNA. Proc. Natl. Acad. Sci. USA 84, 5680-5684. 

Recently, Affymetrix also launched their exon arrays (i.e., arrays that use multiple probes to target essentially every exon of each transcript variant). This platform is the most detailed for analysis of gene expression levels, and it allows for identification of alternative splicing in addition to the standard genome-wide transcriptional analysis. 

Gene expression may also be affected post-transcriptionally by altering mRNA levels. The insulin receptor has at least two splice variants termed type A or B. Exon 11 in the insulin receptor gene is spliced from the mRNA in the Type A variant and is associated with hyperinsulinemia. Over 30,000 alternative splice sites have been identified in a genome-wide analysis of humans, although functional polymorphisms at these sites have yet to be fully characterized. 

Alternative mRNA splicing detection and analysis of human exonic enhancer sequences using genomic SELEX... 

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