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Genetic analysis gene disruption

Mutant or knockout mice defective in specific enzymes involved in lipid synthesis have provided powerful tools for genetic analysis of lipid function in the nervous system. For example, disruption of the genes for ceramide galactosyl transferase or galactosyl ceramide sulfotransferase,... [Pg.44]

Genetic analysis of CYP51 by gene disruption, the first for a CYP in any organism, demonstrated the essential nature of the gene product. Chemical inhibition of CYP activity was also studied and genetic suppression of the effect of azole inhibition (by ketoconazole and fluconazole) was found to be mediated by sterol CS-desaturase mutants. These mutants changed... [Pg.604]

Brakhage A A, Browne P, Turner G (1994). Analysis of penicillin biosynthesis and the expression of penicillin biosynthesis genes of Aspergillus nidulans by targeted disruption of the acvA gene. Mol. Gen. Genet. 242 57-64. [Pg.44]

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