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Gene therapy for kidney diseases

Sifeng Chen,1,2 Kirsten M. Madsen,2 C. Craig Tisher2 and [Pg.161]

ADENO-ASSOCIATED VIRAL VECTORS FOR GENE THERAPY [Pg.162]

Transfection efficacy of naked DNA can be increased by physical methods such as electroporation and sonication. Electroporation employs electric pulses to punch holes in the cell membrane, usually smaller than 10 nm but larger than oligonucleotides. With the use of electroporation, DNA was delivered into the cytosol of cells by diffusion. Since its introduction in 1982, in vivo transfection has been achieved in skeletal muscle, fiver, skin, tumors, testis, and the kidney. Tsujie et al. (2001) developed a method to target glomeruli using electroporation in vivo wherein injection of plasmid DNA via the renal artery was followed by application of electric fields. The kidney was electroporated by sandwiching the organ [Pg.164]

Application of gene therapy for specific kidney diseases 7.5.1. Genetic renal disorders... [Pg.176]

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Application of gene therapy for specific kidney diseases

Disease genes

Disease therapy

Gene disease genes

Gene therapy

Gene therapy for

Kidney diseases

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