Gene search

Sequence comparison with those reported in the protein bank comparison of experimentally determined and predicted MW and pi amino acid sequence to determine function oligonucleotides design for gene search. 

Secondary structure HAIRPN HAIRGU Gene searching TRNA... 

Figure 6.3. Results of an MGD Quick Gene Search for pmp22.

We have examined two complete chloroplast genome sequences (Nicotiana tabacum (12) and Marchantia polymorpha (13)) and some individual chloroplast genes searching for LRE-like matrices homologous to the light responsive elements found in plant nuclear light-inducible... 

The amino acid sequence gives an identity to the protein and provides fundamental information on the nature of the protein, such as structure, mechanism of action, evolutionary origin, and classification into enzyme families. This primary information also serves as a guide for gene search, molecular cloning, and manipulation of genes in recombinant DNA technology. 

DNA sequence information is the starting poiat for other appHcations, including the expression of a gene product, the search for related sequences ia biological samples, in vitro mutageaesis of the sequeace, and stmcture—function studies of gene expression. 

There has been an extensive search for additional opioid receptor genes with homology to p, 8, and k receptors which was, however, unsuccessfiil. It is likely, therefore, that the functional properties of the subdivision of p, 8, and k receptors as well as that of the e and X receptors results from alternate mRNA processing, posttranslational modification of the receptor, and/ or from the formation of homo- and heterodimeric receptor complexes. 

Metagenomics has been applied to the search for novel genes encoding the synthesis of vitamins such as biotin and vitamin C [47,76]. Seven cosmids were detected in metagenomic libraries obtained after avidin enrichment of environmental samples. The highest levels of biotin production in this study were detected in a cosmid... 

Carpita N. Tierney M. Campbell M. (2001) Molecular biology of the plant cell wall Searching for genes that define structure, architecture and dynamics // Plant Mol. Biol. V. 47. P. 1- 5. 

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