Gene expression system advantages

Here, we describe the various alternatives to the use of E. coli expression hosts for heterologous gene expression. Advantages and disadvantages for the different expression systems are discussed, and practical aspects of expression technologies are also described. The feasibility of isotope labeling of recombinantly expressed proteins and their potential use for NMR is also discussed, since the costs and quantities of recombinant proteins produced depend on the system being used. 

Expression of eukaryotic genes in yeast has two main advantages (i) the yeast expression system contains many features of a eukaryotic expression system such as glycosylation or disulfide bond formation, and (ii) yeast is a very economical system. Yeasts are single cells, can be cultivated easily, feature fairly short doubling times, and require relatively inexpensive medium ingredients in many aspects they resemble bacteria. 

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