Gene expression advantages

Here, we describe the various alternatives to the use of E. coli expression hosts for heterologous gene expression. Advantages and disadvantages for the different expression systems are discussed, and practical aspects of expression technologies are also described. The feasibility of isotope labeling of recombinantly expressed proteins and their potential use for NMR is also discussed, since the costs and quantities of recombinant proteins produced depend on the system being used. 

Infection is established Viral genes expressed Viral genome replicated Production of infectious virions blocked No protection from CPE No protection from CTL No selective advantage... 

It is well known that survival and proliferation of microbial cells in the environment depend on the expression of advantageous phenotypes controlled by the genotype expression. It is becoming clear that such evolutionary pressure has resulted in a network of sensor mechanisms that transduce environmental stimuli into gene expression and hence a phenotype complementary to the prevail-... 

Alternatively, gene therapy can be performed through in vivo manipulation of gene expression. In this case, the desired genes must be directly delivered to the appropriate cell population in the subject in vivo. This strategy offers several advantages over the ex vivo approach. For instance,... 

We may also find that Sabatini s reverse transfection method of creating "live cell" microarrays offers even greater advantage for drug discovery and development (Ziauddin and Sabatini, 2001). Their method, relying on arrayed cDNA expression vectors to transform adherent cells, provides localized real-time gene expression analysis of the putative gene product. 

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