Gastric juice, polarography

The presence of peptides and amino acids in gastric juice was demonstrated many years ago by Nencki and Sieber (N3), Babkin (Bl), and Komarov (K22). This became better documented only more recently with the use of modem physicochemical tools, such as paper partition chromatography, electrophoresis, and polarography. Demonstration of the products of proteolytic degradation of serum proteins and muco-substances has also been made possible only by the use of these methods. The reader is referred to the second article by the author (G21) for pertinent information and to the article by Bouda (B29). 

The principle of polarography, developed by Heyrowsk and Brdicka, was applied in studying gastric juice first by Sibata, Kanazawa and Sato (see Kl), followed by Sasai (S1-S3) and Kakei (Kl, K2, K5). 

Sasai et al. (S1-S3), using polarography, found a dialyzable peptide in the filtrate of gastric juice after precipitation with methanol. It was perhaps related to that formed by peptic digestion of the protein... 

Extending Sasai s work, Kakei (Kl, K2, K5) found a high doubleprotein wave on polarography in the majority of anacid gastric juices... 

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