Galacturonic acid antibodies

Agar diffusion and inhibition results for the galacturonic acid antibodies are reproduced in Fig. 20 C and D. The precipitin bands in Fig. 20 C are not as intense as in Fig. 20 A, and the second band between wells S and Ag is missing because the photograph was taken after a short diffusion time. The preparation of anti-D-xylose and anti-D-galacturonic acid antibodies has been achieved for the first time. 

Fig. 19.—Elution patterns of anti-xylose (A) and anti-galacturonic acid (B) antibodies from adsorbents with xylose ligands and with flaxseed polysaccharide ligand. Elution with D-xylose and with D-galacturonic acid indicated by arrows.

Fio. 20.—Agar diffusion and inhibition of antibodies specific for D-xylose units (A and B) and specific for D-galacturonic acid (C and D). 

Quantitative precipitin tests were performed as described.73 The amounts of precipitates obtained at the various concentrations were measured by the protein phenol method.74 The inhibition values are recorded in Table I. These inhibition data show that the precipitin reaction between the tetrahet-eropolysaccharide and the anti-GlcA antibodies is strongly inhibited by d-glucuronic acid, but with the exception of galacturonic acid none of the other carbohydrates were inhibitory. The data in the table also show that the carbohydrates tested did not inhibit the precipitin reaction between the polysaccharide and anti-GlcA-Rha antibodies. The monosaccharides alone cannot completely fit the active site of this antibody to give a precipitin test. 

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